Part I. mRNA Synthesis, Purification, and Transfer

1. Cell Engineering with Synthetic Messenger RNA

Peter M. Rabinovich and Sherman M. Weissman

2. In vitro Transcription of Long RNA Containing Modified Nucleosides

Norbert Pardi, Hiromi Muramatsu, Drew Weissman, and Katalin Karik

3. HPLC Purification of in vitro Transcribed Long RNA

Drew Weissman, Norbert Pardi, Hiro Muramatsu, and Katalin Karik

4. Synthetic mRNAs with Superior Translation and Stability Properties

Ewa Grudzien-Nogalska, Joanna Kowalska, Wei Su, Andreas N. Kuhn, Sergey V. Slepenkov, Edward Darzynkiewicz, Ugur Sahin, Jacek Jemielity, and Robert E. Rhoads

5. Non-Viral, Cationic Lipid-Mediated Delivery of mRNA

James G. Hecker

6. Light-Induced mRNA Transfection

Sigurd Lein's B e and Eivind Hovig

7. Engineering B Cells with mRNA

Jaewoo Lee, David Boczkowski, and Smita Nair

8. Programming Human Dendritic Cells with mRNA

Jaewoo Lee, David Boczkowski, and Smita Nair

9. Large Volume Flow Electroporation of mRNA: Clinical Scale Process

Linhong Li, Cornell Allen, Rama Shivakumar, and Madhusudan V. Peshwa

10. Retrovirus-Based mRNA Transfer for Transient Cell Manipulation

Melanie Galla, Axel Schambach, and Christopher Baum

11. mRNA Delivery to Human Dendritic Cells by Recombinant Yeast and Activation of Antigen-Specific Memory T Cells

Frank Breinig, Tanja Breinig, and Manfred J. Schmitt

Part 2. Modulation and monitoring of cell metabolism

12. Non-Viral RNA Transfection to Transiently Modify T Cells with Chimeric Antigen Receptors for Adoptive Therapy

Tobias Riet, Astrid Holzinger, Jan D rrie, Niels Schaft, Gerold Schuler, and Hinrich Abken

13. Natural Killer Cell Reprogramming with Chimeric Immune Receptors

Noriko Shimasaki and Dario Campana

14. Reprogramming to Pluripotency and Differentiation of Cells with Synthetic mRNA

Peizhe Wang and Jie Na

15. Anti-Tumor Vaccination with Synthetic mRNA: Strategies for in vitro and in vivo Preclinical Studies

Mustafa Diken, Sebastian Kreiter, Abderraouf Selmi, zlem T reci, and Ugur Sahin

16. Mannosylated and Histidylated LPR Technology for Vaccination with Tumor Antigen mRNA

Chantal Pichon and Patrick Midoux

17. Analysis of Surviving-Specific T Cells in Breast Cancer Patients Using Human DCs Engineered with Survivin mRNA

zcan Met and Inge Marie Svane

18. mRNA electroporation as a Tool for Immunomonitoring

Nathalie Cools, Kirsten Van Camp, Viggo Van Tendeloo, and Zwi Berneman

19. mRNA PCR-Based Epitope Chase Method

Jean-Daniel Doucet, Dominique Gauchat, and R jean Lapointe

Part 2. Modulation and monitoring of cell metabolism

12. Non-Viral RNA Transfection to Transiently Modify T Cells with Chimeric Antigen Receptors for Adoptive Therapy

Tobias Riet, Astrid Holzinger, Jan D rrie, Niels Schaft, Gerold Schuler, and Hinrich Abken

13. Natural Killer Cell Reprogramming with Chimeric Immune Receptors

Noriko Shimasaki and Dario Campana

14. Reprogramming to Pluripotency and Differentiation of Cells with Synthetic mRNA

Peizhe Wang and Jie Na

15. Anti-Tumor Vaccination with Synthetic mRNA: Strategies for in vitro and in vivo Preclinical Studies

Mustafa Diken, Sebastian Kreiter, Abderraouf Selmi, zlem T reci, and Ugur Sahin